WebChanges in microscopic viscosity and macromolecular crowding accompany the transition of proteins from their monomeric forms into highly organised fibrillar states. Previously, we have demonstrated that viscosity sensitive fluorophores termed WebFluorescent properties of non-sulfonated cyanines have little dependence on solvent and surrounding. Absorbance and fluorescence spectra of non-sulfonated cyanine dyes are plotted below. Sulfonated cyanines Sulfonated cyanines include additional sulfo-groups which facilitate dissolution of dye molecules in aqueous phase.
Introduction to Fluorescent Labels: Cyanine Dyes Cy7, Cy5, Cy5.5, …
Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while … WebJan 10, 2024 · Alexa Fluor® dyes are a big group of negatively charged and hydrophilic fluorescent dyes, frequently used in fluorescence microscopy. All the Alexa Fluor® … determinants of corporate cash holdings
Cyanine dyes - Lumiprobe
WebCy3 Acid (also sold under Sulfo-Cyanine3 Acid) is a bright, water-soluble, and pH insensitive> from pH 4 to pH 10 orange-fluorescent dye. Cy3 conjugates of antibodies, peptides, and proteins can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. http://www.xarxbio.com/news/news-7-94.html WebActivated fluorescent dyes are routinely used to tag proteins, nucleic acids, and other biomolecules for use in life science applications including fluorescence microscopy, flow cytometry, fluorescence in situ hybridization (FISH), receptor binding … chunky heeled loafer