WebMay 15, 2015 · Flp-In 293 cells (Invitrogen: R750-07) were maintained in DMEM containing 10% FBS. All cells were incubated at 37°C in a humidified atmosphere containing 5% CO 2 . Cultured hTRPA1-expressing cells and Flp-In 293 cells were seeded onto 96-well black-wall plates for 24 h prior to their use in experiments. WebCell information for Flp-In-293. Find diseases associated with this biological target and compounds tested against it in bioassay experiments. This application requires Javascript.

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WebFlp-In 293 cell line is designed for rapid generation of stable cell lines that ensure high level expression of your protein of interest from a Flp-In expression vector. Targeted … WebSep 12, 2024 · The Flp-In™ System (Invitrogen) was used to generate a cell line expressing Halo-HDAC1 under the control of the CMVd2 promoter in Flp-In™-293 host cells essentially as described previously 55 ... kfintech capital gains

Invitrogen™ Flp-In™-293 Cell Line - Fisher Sci

WebI am trying to generate stable cell line by using Flp-In 293 T-REx cells. I use 9:1 (w/w) ratio of pOG44:pcDNA-GOI and lipofectamine as a transfection reagent. WebFlp-In™-293 Human embryonic kidney 1 × 107 cells, frozen R750–07 Flp-In™-CV-1 African Green Monkey kidney 1 × 107 cells, frozen R752–07 Flp-In™-CHO Chinese Hamster … WebNov 1, 2010 · Wild-type and mutant BCRP-expressing HEK-293 or Flp-In-293 cells and the vector control cells were plated in four-chamber polysterene vessels on glass slides (Falcon; BD Biosciences, Bedford, MA) at ∼2 × 10 5 cells/well. Cells were grown to 80% confluence and washed twice with PBS at room temperature. Cells were then fixed with 4% ... kfintech branch address