Cleaved cdcp1 sequence

Author: nqlo

August undefined, 2024

WebJun 15, 2024 · Here, we characterized the precise proteolytic isoforms of CUB domain containing protein 1 (CDCP1), a protein overexpressed and specifically cleaved in RAS-driven cancers. We validated that the N-terminal and C-terminal fragments of CDCP1 remain associated after proteolysis in vitro and on the surface of pancreatic cancer cells. WebApr 15, 2024 · Using a CDCP1-biased probe, we identify urokinase (uPA) as the master regulator of CDCP1 proteolysis, which acts both by directly cleaving CDCP1 and by activating CDCP1-cleaving plasmin.

In vivo cleaved CDCP1 promotes early tumor …

WebJan 9, 2014 · This CDCP1 cleavage-induced signaling cascade constitutes a unique mechanism, independent of extracellular matrix remodeling, whereby a … WebCDCP1 (CUB-domain containing protein 1), also known as Trask or CD318, is a type I transmembrane receptor whose specific role remains unclear. Human CDCP1 is synthesized with a large extracellular domain (ECD) … janesville youth baseball

Blocking of CDCP1 cleavage in vivo prevents Akt-dependent ... - Nature

WebJan 9, 2014 · ABrogation in vivo of CDCP1 cleavage either by unique cleavage-blocking monoclonal antibody 10-D7 or by inhibition of proteolytic activity of plasmin-like serine proteases with aprotinin prevented β1 integrin/CDCP1 complexing and downstream FAK/Akt signaling concomitant with significant reduction of stromal invasion and spontaneous … WebMay 28, 2024 · Full length CDCP1 (135 kDa) and its cleaved form ... (Fasmac DNA Sequence Service, Fasmac Co. Ltd.). To analyze the mutations, exon 20 of the EGFR gene was amplified using the forward primer (5 ... WebWhile CDCP1 cleavage signaling is crucial for tumor cell survival during late stages of metastasis, namely tissue colonization at the secondary sites, cleaved CDCP1 signals for enhanced motility ... lowest phylogenetic species

CDCP1 drives triple-negative breast cancer metastasis …

WebNational Center for Biotechnology Information WebFeb 15, 2024 · Here, we demonstrate that Abs that selectively recognize a proteolytic neoepitope on CUB domain containing protein 1 (CDCP1) could enable more effective and safer treatments for solid tumors. CDCP1 is highly overexpressed in RAS-driven cancers, and its ectodomain is cleaved by extracellular proteases. lowest ph thing we drinkWebFeb 15, 2024 · The cleaved-specific CDCP1 Abs described here demonstrate that specifically targeting c-CDCP1 is effective and has a more favorable safety profile … janesville youth baseball softball

"WebApr 13, 2015 · An in silico analysis of the primary sequence of CDCP1 revealed that the tyrosine residues Y734 and Y743 were both located in an YXXI/L consensus sequence ... It was recently proposed that the cleaved CDCP1 would mainly occur through plasmin in vivo and would have a pro-survival function during metastatic colonization . In our hands, the … " - Cleaved cdcp1 sequence

Cleaved cdcp1 sequence

Recombinant Human CDCP1 Fc Chimera Protein, CF

WebJul 24, 2024 · Importantly, CDCP1 appears to be mostly full-length in PME and MCF10A and cleaved in five of five breast cancer cell lines that express CDCP1. Although … WebAug 5, 2016 · CDCP1 is a single-pass transmembrane protein with three extracellular CUB domains and a short intracellular tail. Tyrosine phosphorylation of the intracellular …

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WebJan 13, 2024 · The results presented here indicate that TA blocks cleavage of the Plasmin substrate CDCP1. Some reports indicate that the cleaved form of CDCP1 exhibits enhanced oncogenic functions and a different spectrum of protein-protein interactions than the full-length protein (Casar et al., 2014; Law et al., 2016; Wright et al., 2016). … WebFeb 15, 2024 · These Abs potently targeted cleaved CDCP1-expressing cancer cells as an Ab-drug conjugate, an Ab-radionuclide conjugate, and a bispecific T cell engager. ... The R368/K369 cleavage site was replaced with Px recognition sequence (GS) 5-LEVLFQGP-(GS) 5. (B) SDS-PAGE of CDCP1 constructs. Px treatment cleaves CDCP1(Px)-Fc into …

WebMar 21, 2024 · GeneCards Summary for CDCP1 Gene. CDCP1 (CUB Domain Containing Protein 1) is a Protein Coding gene. Diseases associated with CDCP1 include Colorectal … WebCDCP1 Antibody detects endogenous levels of total CDCP1 protein. The 140kDa band is full length, glycosylated CDCP1, the 70kDa band is a cleavage product of CDCP1. Species Reactivity: Human Source / Purification

WebWe found that in the absence of cofactor Va, the wild-type sequence at the R271 site is cleaved preferentially regardless of its position at the R271 or R320 site, whereas in the presence of cofactor Va, the R320 site is cleaved preferentially regardless of its sequence. Additional single-molecule FRET measurements revealed that the environment ... WebAug 8, 2024 · Therefore, CDCP1 may form a homophilic complex at the plasma membrane through its ECD. CUB1, the domain located furthest from the transmembrane domain, is cleaved and released, and has been detected in the urine of patients with cancer (18,19). CDCP1 cleavage in the ECD induces cell migration in triple-negative breast cancer cells …

WebDec 19, 2011 · In contrast to the cleaved CDCP1-WT in control embryos, no cleavage of CDCP1, and little or no tyrosine phosphorylation of the 135-kDa CDCP1 were detected …

WebFeb 15, 2024 · Here, the authors generated an antibody that specifically targeted cleaved CDCP1 (c-CDCP1) and developed a drug conjugate, a vector for radioactive ions, … lowest ph to melt waxWebTargeting CDCP1 impairs PDAC cell functions and PDAC tumor growth independently of CDCP1 cleavage status. A CDCP1-targeting antibody is highly effective at delivering imaging radionuclides and cytotoxins to PDAC cells allowing specific detection of tumors by PET/CT imaging and superior anti-tumor effects compared to gemcitabine in in vivo models. janesville youth baseball complexWebFeb 15, 2024 · CDCP1 is highly overexpressed in RAS-driven cancers, and its ectodomain is cleaved by extracellular proteases. Biochemical, biophysical, and structural … janesville youth football janesville wiWebDec 3, 2012 · This CDCP1 cleavage-induced signaling cascade constitutes a unique mechanism, independent of extracellular matrix remodeling, whereby a proteolytically … janesville youth hockey clubWebMar 29, 2024 · The CDCP1 is cleaved by serine proteases at adjacent sites, arginine 368 (R368) and lysine 369 (K369), which induces cell migration in vitro and metastasis in vivo. CDCP1 was the most significant (FDR adjusted p-value 0.006). Correlation with CDCP1 … MT1-MMP is accumulated at invadopodia by targeted delivery via membrane … Kinase activity was evaluated using phosphorylation site-specific antibodies … CDCP1 is phosphorylated by Src family kinases in its full-length and cleaved … Background: Galectins are beta-galactose specific binding proteins. In human … The CUB domain-containing protein-1 (CDCP1) is a transmembrane molecule … janesville youth softballWeb1 day ago · However, in Alzheimer’s and other neurodegenerative diseases, P35 is cleaved into a smaller protein called P25, which can also bind to CDK5 but has a longer half-life than P35. ... They designed their peptide with a sequence identical to that of a segment of CDK5 known as the T loop, which is a structure critical to the binding of CDK5 to P25 janesweatherWebWhile CDCP1 cleavage signaling is crucial for tumor cell survival during late stages of metastasis, namely tissue colonization at the secondary sites, cleaved CDCP1 signals … lowest ph thing